BIOENG168L

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Practical Light Microscopy

BioengineeringUndergraduateCOE - College of Engineering

Subject

BIOENG

Course Number

168L

Department

Course Level

Undergraduate

Course Title

Practical Light Microscopy

Course Description

This laboratory course is designed for students interested in obtaining practical hands-on training in optical imaging and instrumentation. Using a combination of lenses, cameras, and data acquisition equipment, students will construct simple light microscopes that introduce basic concepts and limitations important in biomedical optical imaging. Topics include compound microscopes, Kohler illumination, Rayleigh two-point resolution, image contrast including dark-field and fluorescence microscopy, and specialized techniques such as fluorescence recovery after photobleaching (FRAP). Intended for students in both engineering and the sciences, this course will emphasize applied aspects of optical imaging and provide a base of practical skill and reference material that students can leverage in their own research or in industry.

Minimum

3

Maximum

3

Grading Basis

Default Letter Grade; P/NP Option

Method of Assessment

Written Exam

Instructors

Fletcher

American Cultures Requirement

No

Reading and Composition Requirement

None of the Reading and Composition Requirement

Repeat Rules

Course is not repeatable for credit.

Formats

Lecture, Laboratory

Term

Fall and Spring

Duration (in weeks)

15

Minimum Hours

2

Maximum Hours

2

Minimum Hours

3

Maximum Hours

3

Outside Work Hours Min

4

Maximum Hours

4